mPEG2-NHS

Description

mPEG2-NHS ester: A polyethylene glycol (PEG) derivative containing two methoxy end groups at one end of the PEG chain and one N-hydroxysuccinimide (NHS) ester functional group at the other end of the PEG chain.

• Key Features:
  • PEG (Polyethylene Glycol): PEG is known for its water solubility. The PEG portion of the mPEG2-NHS molecule contributes significantly to its hydrophilic (water-loving) nature and reduces immunogenicity
  • NHS Ester:The NHS ester reacts with primary amines, forming stable amide bonds. However, the overall solubility of the molecule is still largely influenced by the PEG component.

• mPEG2: mPEG2 provides water solubility and biocompatibility and is used to add PEG to other molecules.

• Uses of mPEG2-NHS:
  • It’s commonly used in bioconjugation to modify proteins, peptides, and other molecules containing primary amines.  
    • Protein Modification: mPEG-NHS is frequently used to modify proteins, increasing their solubility, stability, and circulation time. This process, known as PEGylation, can improve the therapeutic efficacy of protein drugs.
    • Surface Modification: It can modify various surfaces, including nanoparticles and other materials, to enhance their biocompatibility or provide specific functional groups.
    • Amine Group Labeling: The NHS ester reacts with primary amine groups (-NH2) on molecules like proteins, peptides, and amine-modified oligonucleotides, enabling labeling or conjugation.
    •  
  • Solubility of mPEG2-NHS:

• • The hydrophilic PEG spacer increases solubility in aqueous media.
  • It is also soluble in organic solvents like DMSO, DCM, and DMF.
    • DMSO (Dimethyl sulfoxide)
    • DCM (Dichloromethane)
    • DMF (Dimethylformamide)