Biotin-PEG-Biotin

Description

Biotin-PEG-Biotin has a biotin end group at both ends of the PEG chain. Biotin-PEG linkers are a product line that combine the high affinity binding properties of biotin with the hydrophilic and flexible characteristics of polyethylene glycol (PEG). These linkers can be used for detection, immobilization, targeting, labeling, and nonradioactive purification.

Key Features of PEG-Biotin

• Strong biotin-avidin binding: Biotin exhibits an exceptionally strong and specific affinity for avidin and streptavidin.
• PEGylation: is the process of attaching PEG to a molecule, often enhancing solubility, stability, and reduces immunogenicity. Biotin-PEG linkers can be used to introduce PEG moieties to proteins or other molecules.
• Protein immobilization: These linkers can be used to immobilize proteins or other molecules onto surfaces functionalized with avidin or streptavidin.

Applications of Biotin-PEG Linkers:

• Immobilization: Immobilizes proteins, enzymes, or other biomolecules onto surfaces for various applications.\
• Immunoassays: Biotin-PEG linkers are essential components of many immunoassays, such as ELISA (Enzyme-Linked Immunosorbent Assay) and Western blotting.
• Targeting: Delivers molecules to specific cells or tissues by targeting biotin-tagged receptors.
• Labeling: Labels biomolecules for visualization or tracking.
• Nonradioactive Purification: Purifies proteins or nucleic acids based on their biotinylation.

Chemical Properties

• Biotin conjugation: The biotin is typically conjugated to the PEG chain through a stable amide bond.
• PEG chain: The PEG chain can vary in length and can be linear or branched.
• Solubility: generally soluble in water, aqueous buffers, chloroform, methylene chloride, DMF, and DMSO. Solubility may be limited in alcohols, toluene, and ether.